Saliva is a clinicaly important biologic fluid. The present study aims at determining the role of saliva in detection of pregnancy using home- based pregnancy detection kits to provide an alternative biofluid that is more user friendly, acceptable, non- invasive, rapid and easy for home use. Materials and method: The study was conducted among 100 women visiting a gynecology clinic for confirmation of pregnancy with a history of a missed menstrual cycle not more than 4 weeks. The test will be performed using pregnancy detection kit with 20 mIU specification for estimation of salivary hCG. Routine tests was prescribed to the patients such as laboratory based urine hCG and/ ultrasound examination was used for confirmation of pregnancy status & correlation with the saliva based result. Results: About 84% participants with a confirmed pregnancy showed a positive result, of which 62% showed a dark band & a light band was noted in 22% participants. A negative results was noted in 16% participants & were positive for urine test result. Conclusion: The result of this study showed that traditionally used biofluid can be replaced with more user friendly & acceptable biofluids. i.e,saliva as a biomarker for detection of pregnancy. In this study urinary hCG based kit was used but it opens new avenues towards development of new Salivary based diagnostic kits for detection of pregnancy.