Biomedical waste demarcates one of the most common waste components which is emerging on an alarming rate especially due to a rapid increased Healthcare sector and an increase in number of healthcare facilities in India. It has been a greater concern to find out some generous methodologies to systematically monitor the accumulated wastes in facilities and also it has become quite important to develop processes for decomposing and degrading these Biomedical wastes. Within this contrast an important concern is degrading a huge number of cellulosic wastes which could be in form of Cotton swabs, Dressing Bandages, Tissue Papers and other wastes. As the main component of these basic Biomedical wastes is Cellulose, so the prime focus of the work hereby is to screen the Microbes which are capable of Producing Bacteria and also Optimizing the medium for enhancing the cellulose activity. Among the 5 isolates taken it was found that only 2 isolates i.e., CL 002 and CL005 were showing significant selectiveness for Cellulase Producing Bacteria as per the results obtained