.

ISSN 2063-5346
For urgent queries please contact : +918130348310

Screening of pea (Pisum sativum L.) accessions for drought stress tolerance based on leaf disc assay and stomata density

Main Article Content

Anamika, Raghvendra Saxena*, R.S. Tomar, Raghvendra Kumar Mishra*
» doi: 10.48047/ecb/2023.12.10.422

Abstract

Pea is one of the most significant protein-rich pulse crops grown in various regions of the world; thus, it is an important member of the agroecological cropping system. Several morphological and physiological parameters, such as seed germination, plant height, root length and weight, total biomass, flower development, photosynthesis rate, chlorophyll content, and antioxidant activity, directly depend on the potential of particular genotypes/accessions. The objective of the present study was to identify drought-tolerant pea accession based on leaf disc and stomata density assay. Leaf disc and stomata density assays have some advantages over whole-plant assays because they require less sample preparation time without harvesting of plants and allow a higher number of replicates. Leaf samples of fifteen pea accessions and two commercial varieties were used for leaf-disc and stomata density assay. Drought condition was generated by mannitol (100mM and 150mM) in the laboratory to check stress-tolerant pea accession. After one week, it was observed that two accessions ARG6 and ARG10 were stress-tolerant, as the colour of the disc remained green in 150mM and 200mM mannitol in compared to other pea accessions. Furthermore, low stomatal density was observed in

Article Details