Five anthocyanin derivatives were identified in the fruit of Rubus ellipticus (Hisalu) by using advanced analytical techniques, HRLC-ESI-MS/MS. Five major anthocyanin derivatives of cyanidin, petunidin, and delphinidin were extracted from the fruit of Rubus ellipticus (RE) and relative concentrations of the identified anthocyanin derivatives of petunidin, delphinidin, cyanidin were found to be: 0.18 ± 0.02 mg/ g, 0.26 ± 0.02 mg/ g and 0.058 ± 0.02 mg/g fruit weight respectively, and for cyanidin-3-galactoside/glucoside and for petunidin-3-O-glucose the relative concentration was found to be 0.078 ± 0.02 mg/g fruit weight. Total anthocyanin content (TAC) was found to be: 0.58 ± 0.02 mg/g fruit weight. The content of anthocyanin was found to be higher in the fruit of Rubus ellipticus compared with Prunus carmeniaca, Pyracantha crenulata, and Celtis australis fruits.