Volume - 13 | Issue-1
Volume - 13 | Issue-1
Volume - 13 | Issue-1
Volume - 13 | Issue-1
Volume - 13 | Issue-1
Ferulic acid and Quercetin are vital phenolics that are commonly obtained in the leaves, fruits, and seeds of plants. They have the potential against cancer proliferation, chronic inflammation, antiviral, and various other oxidative manifestations. Therefore, the high-performance thin-layer chromatography (HPTLC) method has been established for the simultaneous identification of quercetin and ferulic acid. The aqueous and ethanolic extract of Sida cordifolia, a high source of ferulic acid and quercetin, was employed in this investigation. These bioactives were magnificently analyzed at the wavelength of 254 nm using mobile phase Toluene: Ethyl acetate: Formic acid (5:4:1 v/v/v). Further, the method was validated and verified as per the ICH guidelines. This simultaneous identification method of bio-actives will prove to be accurate, rapid, cost-effective, and time-saving for many scientists.