Aim and Objectives: Male infertility comprises more than one third infertility problems globally. It has been postulated that sleep hygiene can affect semen parameters such as total count, concentration, and motility. Also, Persian traditional medicine has long advocated the importance of sleep hygiene on general health and reproductive system, hence, this systematic review aimed at evaluating the relationship between sleep hygiene and male infertility from the perspective of Persian traditional medicine. Material and Methods: A systematic search of literature was performed in PubMed, Scopus, Web of Science, IranMedex, and SID from inception to April, 2023 with relevant keywords. Those studies that investigated the role of sleep hygiene on semen parameters and male reproduction have been included. Textbooks of Persian medicine has been used accordingly. Results: After screening, 15 studies have been added in our systematic review. According to the results of the included studies, poor quality of sleep is significantly associated with diminished total sperm count, sperm concentration, sperm motility, and sperm morphology. Five studies reported no significant association between sleep hygiene and semen parameters. The most used sleep questionnaires among the included studies were Karolinska sleep questionnaire and Pittsburgh Sleep Quality Index. Due to high heterogeneity of the included studies and heterogenous comparison of the results, meta-analysis could not be performed. Conclusion: Several studies have indicated the significant association between sleep hygiene and sperm count, concentration, motility, and morphology. The Persian traditional medicine has constantly emphasized on good nutrition and sleep, based on the necessary sixes (the common method for prevention of diseases in Persian medicine) and this method for lowering infertility among males have been disregarded in the literature. This accurately highlights the potential of Persian traditional medicine in prevention and treatment of male infertility. Further studies are needed to elucidate the precise relationship and underlying mechanism.