Recently, the increase of the global population, the loss of arable land, soil erosion, and the conversion of agricultural land into urban areas due to uncontrolled development have all put pressure on crop productivity. Artemisia annua L., is an annual plant local to Asia and Eastern Europe, is the primary source of the antimalarial chemical compound artemisinin, a sesquiterpene alkaloid found in maximum amounts in the leaves. Artemisinin, a bioactive compound present in the leaves of Artemisia, is thought to be an effective drug for malaria treatment. Under the stress of NaCl (sodium chloride), a randomized-design pot experimentation was undertaken with A. annua species. Plants were subjected to increasing soil-applied NaCl concentrations. All plant measurements were conducted 90 days prior to flowering. According to the results, A. annua plant showed a significant to NaCl stress; nonetheless, the highest dose of NaCl concentration was the most damaging to A. annua plants. It dramatically decreased plant growth and leaf chlorophyll 90 days after planting (DAP). However, the intermediate treatment has greatly enhanced the pre-flowering physiological and biochemical activities of A. annua plants, demonstrating that NaCl mediates oxidative stress. Intriguingly, both artemisinin concentration and production enhance dramatically with increasing NaCl concentrations.