AIM- The aim of the present investigation is to isolate, characterize and develop chromatographic analysis of dichloromethane extract of Dolichandrone falcate Seem. leaves. MATERIAL & METHODS- The correctly identified plant leaves is dried in shade at room temperature & after 4-5 days, it is formed in powder by mixer grinder. Powdered drug 100gm was weighed and packed in soxhlet. Defatted drug was subjected to extraction with dichloromethane, ethyl acetate, ethanol and finally by using aqueous system. TLC for the separation of various bioactive compounds from bioactive extract, dichloromethane was developed to find out the probable number of compounds present in them. Solvent systems; n-hexane: chloroform: ethanol (5:3:1) were found to be most satisfactory. For the HPTLC analysis, The sample was dissolved in 10 ml of dichloromethane, sonicated for 10 minutes, filtered and applied on TLC plates (5×10 cm) in 2 tracks (4μl for dichloromethane extract) in the form of band. One side of twin trough chamber was charged with the solvent system n-hexane: chloroform: ethanol (5:3:1) and allowed to equilibrate for 10 minutes. Plate was then scanned at 366 nm in case of dichloromethane extracts. In column chromatography, mixture as prepared above was fed very slowly into the column without disturbing the silica bed. Thereafter appropriate solvent system i.e. n-hexane: chloroform: ethanol (5:3:1) was poured into the column for elucidation of components. Elute was collected at the rate of 20 drops per minute and each fraction was about 25 ml. RESULTS- The spotted plate was then dipped in mobile phase and solvent front was allowed to travel about 70-80% distance on the plate vertically. The compound I and II is white crystalline needle like substance whereas compound III is off-white. The melting point of Compound I, Compound II and Compound III was 133°C, 176°C and 160°C respectively. Mass spectrum of Compound I, II and III showed a parent molecular ion [M+H] + peak at m/z 414, 412 and 426 respectively which corresponds to the molecular formula C29H50O, C29H48O and C38H64O2. CONCLUSION-Total three compounds were isolated in significant amount and their characterization was done using different spectral techniques based on the following deliberations, to reveal their identity as steroids and triterpenes.