A modified simple, selective, rapid, precise high performance liquid chromatography method has been developed and validated for the estimation of sparfloxacin. The separation was made in a HiQSil C-18 column using aqueous solution of 1% acetic acid and acetonitrile (75:25, v/v) as mobile phase at 290 nm using JASCO UV- 4075UV-VIS Detector and ChromNAV CFR chromatography software ( version 2.0). The mobile-phase flow rate and the sample volume injected were 1.0 ml/min and 20 μl, respectively. Retention time of sparfloxacin was found to be 2.28minutes respectively. The correlation coefficient of sparfloxacin was found to be 0.9996. The method was validated according to ICH guidelines with respect to linearity, accuracy, precision, robustness, specificity, etc. The developed method can be used for routine analysis of sparfloxacin .