Bupivacaine hydrochloride is an anesthetic with prolonged duration of action. The aim of this study was to develop a fast, simple and sensitive spectrophotometric method for the determination of bupivacaine in pharmaceutical preparation. The maxim absorbance was finding at 262 nm. At the same time, a validation study was conducted for the proposed method, in accordance with the bioanalytical method validation guidelines. The studied validation characteristics showed that the proposed method can be successfully applied to pharmaceutical preparations analysis