It has been proposed that the mechanical force of centrifugation produces reactive oxygen species (ROS) that compromise the functional and structural integrity of sperm cells. Many oxygen radicals produced by spermatozoa and leukocytes affect the likelihood of sperm-oocyte fusion during repeated centrifugations. Thus, it is preferable to separate the sperm from the other components using techniques like the swim-up approach or micro swim-up approach. Methods: 400 couples were classified and grouped as follow: Group 1: prepared by standard swim-up approach which split into two Subgroups (include 100 normal male factor and 100 abnormal male factor). Group 2: prepared by mini swim up technique using sperm gradient media which divided into two Subgroups (include 100 normal male factor and 100 abnormal male factor). Results: Pregnancy rate was higher (42.0%) in mini swim up group (normal male factor) compare to (41.0%) in swim up group (normal male factor), (46.0%) in mini swim up group (abnormal male factor) compare to (36.0%) in swim up group (abnormal male factor. Conclusions: Sperm preparation has been reported to result in the enrichment of sperm with intact chromatin, which is anticipated to enhance the odds of generating a healthy pregnancy, even though both swim-up and density gradient approaches have been proven to be successful in creating a sperm population with low proportion of apoptotic sperm.