Sphaeranthus indicus Linn, in the Ayurvedic medical system, is used in different dosages and preparations to treat a variety of ailments, including tuberculosis, lung conditions like bronchitis and asthma, indigestion, spleen disorders, elephantiasis, leukoderma, vomiting, urinary discharge, hemicrania, epileptic convulsions, blood purifier, as a poultice for rheumatic pain, aphrodisi. Objective: To study and standardization and anti-microbial activity of Gorukmandi. Methods: After collecting herbs, the extract of Gorukmandi was prepared hydroalcoholic procedure. Then organoleptic, Physico-chemical analyses were done, along with the Standardization method – a chemical with HPTLC method, Heavy Metal Analysis, Anti-Microbial Activity, Total antioxidant by DPPH method, Total phenolic, Total Flavonoid, were performed. Results: The HPTLC studies have shown that it is more versatile than ordinary TLC methods, Heavy Metal analysis showed a minimum presence of metals, antioxidants, polyphenols, and flavonoids. The anti-microbial activity shows that the hydroalcoholic extracts inhibited good antimicrobial activity against all tested fungi and bacteria Conclusion: It is a conclusion that the standardization and potential antimicrobial activity of the extracts from Gorukmundi against tested bacteria and fungi. Further investigation work is to characterize the bioactive compound and other values for fixing standards for this plant