Standardization is an effort to improve product quality and safety. Standardization of pharmaceutical ingredients and drug formulations is a prerequisite for ensuring the reproducibility of drugs and therapeutic quality. With standardization, it is hoped that it can further increase trust in medicines derived from natural ingredients. Standardization of medicinal materials includes starting materials, intermediates, or finished products. Pineapple sea cucumber is one of the natural ingredients that can be used as raw material for herbal medicine. Protein is the main content of pineapple sea cucumber which is often used as an herbal medicine that can help the wound healing process. This study aims to standardize the simplicia of pineapple sea cucumber (Thelenota ananas) native to Pelapis Island, West Kalimantan. Standardization is based on physical, chemical, and microbiological parameters such as raw material requirements for standardized herbal medicines (OHT). The test is performed in the laboratory using the method according to SNI. The results of the Simplicia test of pineapple sea cucumbers from Pelapis Island, West Kalimantan, include water content of 9.46%, protein 59.8%, zinc 0.040 mg/kg, cadmium < 0.054 mg/kg, copper < 0.006 mg/kg, lead 0.001 mg/kg, mercury < 0.007 mg/kg, ALT 8.6 x 102 colonies/g, E.Coli < 3 APM/gr and Salmonella negative met the requirements