As natural resources become more difficult to come by, herbal combinations gain significance. The active phytochemicals present in polyherbal formulations may contribute to their efficacy through the synergistic interaction of active compounds derived from many plants. An essential Ayurvedic polyherbal composition, Ayurgreen Natura Pain Gel is made from the dried and fresh aloe vera plant components, as well as frankincense, myrrh, ferula, and asafetida. Liquid chromatography-mass spectrometry was used to analyze the phytochemistry of Ayurgreen Natura Pain Gel, while Fourier transform infrared spectroscopy and ultraviolet-visible spectroscopy were used to identify the gel's bioactive functional groups