The main purpose of this study is to examine the acute and sub-chronic toxicity studies of chloroform and methanol extract of Eranthemum capense Linn (Acanthaceae) on animal models as per the OECD guidelines 407 & 425 respectively and In-vitro antioxidant activities with standard methods. In acute oral toxicity study a single oral doses of 2000 mg/kg body weight of the individual chloroform and methanol extracts was given to rats and observed them for 14 days for the detection of acute changes and for its mortality any. During acute oral toxicity study period no mortality were observed with no signs of acute changes. Based on these acute oral toxicity studies we performed the sub-chronic toxicity study, in this the chloroform and methanol extract of Eranthemum capense Linn separately given daily at doses