Organic nonlinear optical singe crystal of L-Alanine was grown by low temperature solution growth using water as solvent at room temperature. From the single crystal X-ray diffraction analysis, it crystallises in an orthorhombic system with space group P212121. Various functional groups were identified by Fourier Transform Infrared Spectroscopic analysis (FT-IR). UV-Vis absorbance spectra shows that the lower cut off wavelength was observed at 240nm. Scanning Electron Microscope (SEM) studies reveals the surface morphology of the title material. The Laser Damage Threshold (LDT) for the grown crystal was tested using Nd : YAG laser. The Second Harmonic Generation (SHG) studies shows that the emission of green radiation confirms the NLO behaviour