Varroa mite is considered one of the most damaging pests of honey bees in India as well as worldwide. There are mainly two species of Varroa mites, i.e., Varroa destructor and Varroa jacobsoni, reported from India. The present research is done to analyse the morphometric characters of Varroa mites infesting Apis cerana and Apis mellifera colonies in southern India. So far, no morphometric studies was done to identify Varroa mites from southern India. In this study, morphological investigations were done on different populations of mites collected from five districts. Seven characters namely, body width, body length, width of genital shield, length of genital shield, width of anal shield, length of anal shield and body size ratio were recorded. Mites collected from A. mellifera is having higher body length and body width than mites collected from A. cerana. The body size parameters showed a mean (± SD) body length ranging from 1.661 ± 0.09 to 1.710 ± 0.03 and a body width ranging from 1.113 ± 0.05 to 1.134 ± 0.02 in colonies of A. mellifera. A mean (± SD) body length of 1.430 ± 0.05 to 1.470 ± 0.02 was observed in mites collected from A.cerana colonies. The body size ratio (ratio of body width to body length) of all the collected specimens was greater than 1.4 in both A. mellifera and A. cerana mites