Polyherbal preparations are presently used for treatment of different diseases. In this study polyherbal syrups of extracts of leaf juice of three selected plants were studied for their antidiabetic potential basing on the use by the tribal people and local vaidyas. Clerodendrum infortunatum Linn.(Lamiaceae) has been used against convulsion, diabetes, microbial infections, pain, inflammation. Paederia foetida Linn. (Rubiaceae) has been used against rheumatism, diarrhoea, diabetes, cough, liver diseases, pain, ulcer, microbial infections. Euphorbia neriifolia Linn. (Euphorbiaceae) has been used against CNS stimulation, abdominal sepsis, liver diseases, diabetes, cancer, inflammation, arthritis and skin diseases etc. In this study, the extracted juice of leaves was subjected to drying and concentrated to get a dry mass. The dry juice mass was fractionated using methanol(70%) after defatting with petroleum ether(60 oC – 80 oC). Dry mass of juice and Methanol(70%) extract of each plant were investigated. Phytochemical screening revealed that the extracts contain flavonoids, tannins, sugar, saponins, steroids, terpenoids and phenolic compounds. All extracts were investigated for antidiabetic activities. Non sugar syrup was prepared and evaluated. Formulated herbal syrups were studied towards antidiabetic activities on animals after inducing diabetes by alloxan and streptozotocin and compared with the standard drug glibenclamide. Syrup of Methanol(70%) extract at 300mg/kg of each plant showed highest activity than the corresponding dry mass which may be due to presence of high content of phytochemicals. Polyherbal syrup with the Methanol(70%) extract of plant-I, II & III in the proportion 30:40:30 showed better antidiabetic activity