Vacuum assisted Closure (VAC) dressing produces a localized hypoxia and thereby it helps in wound healing, but the molecules (HIF 1 alpha and VEGF) involved in healing process have not been studied yet in humans however studied in-vitro and in-vivo. Present study was aimed to study association of hypoxia inducible factor 1 alpha in wound fluid with clinical outcome in diabetic patients. Material and Methods: Present study was single-center, prospective, observational study, conducted in patients of age 18-80 years, either gender, with diabetic foot ulcers, undergoing Vacuum Assisted Closure therapy. Wound fluid sample of 5-15cc was collected after application of VAC on day 1, day 3 and towards the end of VAC removal to study effects of HIF 1 alpha and VEGF. Results: In present study, among 35 patients satisfying study criteria, majority were male (85.7 %) & mean age was 58.46 ± 10.93 years. Before VAC treatment, majority patients had Bates-Jensen wound category of extreme (74.3 %) followed by moderate (25.7 %). While after VAC treatment, majority patients had BJ wound category of moderate (45.7 %) followed by extreme (42.9 %), mild (11.4 %). In our study we found that correlation between the parameters HIF 1 alpha (before VAC removal) (ng/ml) & VEGF before VAC removal shows a Poor Positive correlation( p = 0.718), with BJ Wound Score after VAC showing a poor negative correlation with both HIF 1 alpha and VEGF (p=0.987), (p= 0.316), with VEGF being higher in BJ wound category before VAC-extreme group ( p =0.488), though not statistically significant. In our study, BJ score before VAC & HIF 1 alpha day 3 showed a Moderate Negative correlation, being statistically significant in long standing diabetic foot ulcer patients, resulting in stabilization of HIF 1 alpha. Conclusion: Effects of HIF 1 alpha relating with VEGF, an angiogenic factor. Although not very large, the BJ wound result appraisal scale was also associated with the wound liquid proteins.