Hypoglycemia, a prevalent metabolic disorder observed in newborns, can manifest with or without symptoms. The glucose oxidase method is specific and accurate for measuring glucose, it may take some time for result so that they can be utilised for rapid and effective treatment. Glucometers offer several advantages, including simplicity, requiring less blood, quick readings, and the ability to be used by unskilled personnel. Objective: To evaluate the effectiveness, dependability and accuracy of glucometers in estimating blood glucose levels in high risk newborns. Methods: The Department of Pediatrics and Department of Obstetrics and Gynecology carried out a hospital-based cross sectional study at tertiary care hospital, Amravati from October 2021 to April 2023 after ethical approval was taken from scientific community. The study included 100 neonates from the Department of Pediatrics, post natal ward (Obstetrics) and high risk neonates admitted to NICU. Results: Out of 100 cases, 34% of cases were investigated for hypoglycemia by glucometer and 37% of cases were investigated with the laboratory oxidase method. There was a robust positive association observed between the mean glucometer value and the diagnosis by laboratory method value. For the diagnosis of hypoglycemia in neonates, we found an accuracy of around 87.50% for glucometer when we compared with oxidase methods. However, accuracy, detective rate, positive predictive value(PPV) and negative predictive value(NPV) were found to be 97.30%, 94.20%, 95.90 % and 91.80% respectively. Conclusion: The utilization of glucometer for blood glucose estimation is a reliable approach for the detection of hypoglycemia in newborns.