The present ‘in vivo’ study has been designed regarding efficacy of Goghruta as a first aid measure in Common cobra and Russell’s viper snake venom poisoning. The Goghruta for experimentation was of AGMARK standards of Special Grade and snake venom was procured from Haffkine Serum Institute, Mumbai. In animal experimentation study, both the control groups injected respective venom and Goghruta given orally to trial groups. Each group having six albino mice. Mice were observed upto 7 days and observations recorded. In trial group, delay in the duration of appearance of symptoms i.e. tremors, paralysis, convulsions, bleeding and increase in the duration of survival period in comparison with control group is noted. The results of survival period in Russell’s viper venom group was proved statistically significant whereas survival period in Common cobra venom group was nearly significant. The interaction with PVASVS is not noted. Thus, the Antiophidian property of the Goghruta as first aid measure is confirmed by ‘in vivo’study.