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ISSN 2063-5346
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STUDY OF FLAVONOIDS ON THE SURFACE OF SCUTELLARIA LEPTOSIPHON PLANT

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Rustam Muradov, Normurot Fayzullayev, Shavkat Abdullayev, Uchkun Norkulov, Ismail Ergashev, Sunnat Muhibulloyev
» doi: 10.31838/ecb/2023.12.1.041

Abstract

Scutellaria leptosiphon Juz. It consists in studying the flavonoids of the plant and determining their chemical structure. Methods: to extract flavonoids from the plant, we extracted the plant in alcohol and butanol and divided the obtained extractions into fractions. We diluted the alcohol obtained as a result of extraction with distilled water and extracted it with ethyl acetate in a separating funnel. As a result, we separated the fraction with ethyl acetate. In the approximate determination (identification) of the number of flavonoid compounds in the extracts prepared from plants and their authenticity, the distribution (separation) chromatographic method (on paper - PC and a thin layer - TLC) was widely used. Spectrophotometric methods are used to determine the number of flavonoids in the product. We used the photoelectric colourimetric method in the laboratory. Results: S.leptosiphon plant was first collected from the Urgut mountains and its above-ground part was studied it was found that it contains Norvogonine flavone and its glycoside. Conclusions: Scutellaria leptosiphon Juz. practical works such as extraction, separation into fractions in solvents, paper chromatography, thin layer chromatography, and qualitative reactions were performed to extract flavonoids from the surface of the plant. The composition of the sum separated with butanol Norvogonin 7-O-β-D-glucopyranoside was found. The substances obtained as a result of the experiments gave the results of the qualitative chromatographic analysis by the test substances, and it was the basis for our conclusion that their structure is the same as these substances. We believe that the number of substances contained in the studied plant extract, the adequacy of the raw material base, and the high pharmacological activity of this plant in the future, can be used in the field of pharmaceuticals.

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