Forensic pathologists suffer glitches, when it comes to deal with amputated bony remnants of human’s lingering parts such as hand and foot for a medico-legal investigation to conceal the identity, gender, and age of the victim. Among various parameters of identification, one of the criteria in differentiating gender is by measuring second and fourth finger length. Many studies have proved that the length ratio of the second digit to fourth digit (2D:4D) helps to reveal gender, as length of second digit is more in females then in males (2D:4D>1) and the second digit found comparatively shorter than fourth digit in males. The present study has been conducted to investigate the sexual dimorphism by evaluating index and ring finger’s length ratio in North Indian population. Materials and methods: This study was conducted on 408 healthy adults (204 males and 204 females). Index & ring finger length of right & left hand were measured using digital vernier caliper. After collection of data appropriate statistical analysis were done. Result: The ratio of index and ring finger is 0.97mm in males and 0.98mm in females’ right hand and 0.98 mm in males and 0.99mm in females for left hand, however the ratio of index & ring finger length of females was higher when compared to the males. Conclusion: The present study is significant for sex determination and also plays an important role in medicolegal cases & anthropological studies.