The most prevalent mechanism of carbapenem resistance is the development of metallo-β lactamase. Metallo- β lactamase is an Ambler class B zinc-dependent enzyme that can hydrolyze all β lactam antibiotics, including carbapenem. Objective: The purpose of this study was to identify metallo β lactamase producing pseudomonas aeruginosa and antimicrobial resistance pattern isolated from various clinical specimens at a tertiary care hospital in Udaipur, Rajasthan. Methodology: In this study we have analyzed 1000 samples from various clinical samples (Urine, Endrotracheal, Tracheostomy, Pus, Sputum, and C.S.F) and isolate 625 samples of pseudomonas species. Out of 625 samples 506 samples are of Pseudomonas aeruginosa. These 506 samples were further processed for detection of extended spectrum beta lactamase, metallo beta lactamase and AMPc beta lactamase. Result: Out of total 506 samples, we found 151 (29.84%) samples were positive for the extended spectrum beta lactamase (ESBL), 61 (12.05%) samples were positive for metallo beta lactamase (MBL) formation and 125 (24.70%) samples were positive for AMPc beta lactamase. Highest resistance were observed against gefotaxime (55.53%), tigycycline (54.94%) and ceftazidime (51.97%). Conclusion: A routine MBL production test and further research for new antimicrobial drug (which should be less harmful) are needed