Soil is an important component for all terrestrial ecosystem as well as main source of production in agriculture. Enzymes are the vital activators which play an important role in recycling of nutrients and maintaining soil health. Pesticides are the chemical agent that comes in routine in India to increase soil productivity. It have been reported that pesticides pollutes air, water and soil health. The present study deals with the effect of pesticides (such as atrazine, paraquat and fluridone) on enzyme activities of amylase and phosphatse soil enzymes in selected soil from different region of Kannauj U.P. (Tehsil: Tirwaganj, Chhibramau and Kannauj) in winter season. In the present study, It was observed that an effect of pesticides on enzyme activity of amylase and phosphatase mildly decreased as compared to control soil in selected region of Kannauj (U.P.).