Background: Preeclampsia is a pregnancy specific syndrome involving vasospasm and endothelial activation. It is a global problem affecting at least 10% of all the pregnancies. It is a multisystem progressive disorder and is among the leading cause of maternal and perinatal morbidity and mortality. Hypertension and proteinuria are the most prominent features of Preeclampsia. Elevated levels of Homocysteine during gestation have been linked to adverse outcomes in pregnancy like Preeclampsia. Folate and vitamin B12 status has also been related to the occurrence of neural tube defects. Aim: The present study was planned to evaluate the levels of Serum Homocysteine, Folic acid and Vitamin B12 levels and their association in patients with preeclampsia. Methodology: 50 pregnant females diagnosed with preeclampsia were enrolled for the study based on predefined inclusion and exclusion criteria. Age matched Normotensive pregnant women (n=50) constituted the control group. Blood Samples were collected and analyzed for serum Homocysteine, Folic acid and Vitamin B12. Results obtained were analyzed by applying suitable statistical tests. Result: Serum Homocysteine levels were significantly higher in the preeclamptic group as compared to control group (p<0.0001) whereas Serum Folic acid and Vitamin B12 levels were significantly lower. A highly significant correlation (r = -0.469; P<0.0001) was observed between serum Homocysteine and Vitamin B 12 among the preeclamptic group.