Our aim in the present study was to evaluate the association between bone mineral density in lumberspine and femoral neck with serum levels of vitamin D in females of Kanpur, Uttar Pradesh, India, aged between 25 to 65 years. Recent attention to the high prevalence of osteoporosis and its association with low vitamin D levels in adults has raised the importance of vitamin D evaluation. A low level of vitamin D is considered to be one of the most important risk factors for osteoporosis. The present study was a cross sectional study carried out for a period of 1 year. i.e, April 2022 to April 2023. A total of 200 females without any diagnosed chronic disease attending the outpatient department at Rama Medical College Hospital and Research Centre, Mandhana, Kanpur, India, were recruited randomly were included in the study. The serum levels of total Vitamin D were taken to be severely deficient if lower than 20 ng/ml, mild to moderate deficient if it was between 20 and30 ng/ml and optimum level if it was 31–100 ng/ml. We observed that6.6% ,17.1% ,26.7% and 20.0% women suffered severe deficiency of Vitamin Damong the age groups A (25-35 years old), B (35-45 years old), C(45-55 years old) and D (55-65 years old) respectively. While, 30.0%, 67.14, 69.3 and 72.0% had mild deficiency, whereas, 63.3%, 15.7%,4.0% and 8.0 of female among the same groups possessed optimal level of Vitamin D. Overall 20% women suffered from severe, 63% had mild to moderate while 17% showed normal levels of Vit D. And these results indicated that the age group C (45-55 years) had a slightly higher percentage of Vit D deficiency. Only 4-8% of women above the age of 45 had normal Vit D levels. The BMI in all groups showed a significant correlation with the mineral deficiency and osteoporosis. Out of the 200 women included in the study 122 suffered from Osteopenia while 78 had normal bone density. The highest incidence of osteopenia was seen in the Group C (55-65 year) i.e76% while the lowest observed in group A (25-35 years) which was 30.0% among the respective groups. The females with a higher BMI had lower levels of osteopenia and no subject had indications of osteoporosis across the four groups which is a promising indication of better dietary status with respect to Vitamin D.