Tuberculosis disease is chronic disorder and caused in worldwide which 10th is leading death in the world surpassing HIV/AIDS.1 Approx 1.3 to 1.5million people died on every year. Many researchers have done so many molecules and working continuously. The most potent molecule was found from scorpion Diplocentrus melici ,there two types of molecules found first one 1,4 benzoquinone also known as blue benzoquinone and 1,2 bezoquinone also known as red benzoquinone, according to research the blue one have strong anti TB as well as anti MDR and red one showed only anti TB properties. We have taken idea from this molecules blue benzoquinone for working on both site of action. Gated the most superficies enzymes involve for caused mycolic acid synthesis and MDR also. We got some enzyme that is good for binding in docking software. PDB id for both enzymes is 3ZXV and 2Z9i.The first line drug found drug resistance isoniazide, rifampin, also in some time of fluoroquinolone drugs, in second line drugs like amikacin, kanamycine. 3ZXV is the Mycobacterium tuberculosis Glutamine synthetase and 2Z9i is proteolytically active. These two enzymes where found in tuberculosis bacterial function after getting infection and this two enzyme can elaborated the solving problem for bactericidal activity and multi drug resistance. 23 3ZXV pdb id which is targeted on MtGS (Mycobacterium tuberculosis glutamine synthetase), responsible for cell wall synthesis of M. tuberculosis, the parabenzoquinone molecule binds with 3ZXV receptor and getting that good respond on the bases of molecular docking result. Some drugs are already given in tuberculosis that is not treatment effects at the time and some potential drugs having resistance.