A series of novel 2-anilino-N-(2,4’-dioxo-1,2-dihydrospiro[indole-3,2’-[1,3]thiazolidine]-3’-yl) acetamides VII-(a-j) hybrids were synthesized by conventional method via Schiff’s base and cyclisation mechanism. The synthesis of novel targets with antimicrobial and antiviral activities is a core objective today in the context of the COVID-19 pandemic situations. All synthesized structures were confirmed by FT-IR, 1HNMR, LC-MS, and 13CNMR spectral data and the percentage yield of the title compounds was between 50-95%. The newly synthesized hybrids were assessed for their antioxidant activity by DPPH method and neuroprotective activity (the Parkinson activity) by using SH-SY5Y cell lines through MTT assay method. The damage to SH-SY5Y cell lines by 6-OHDA (6-hydroxydopamine) is an established cellular model of Parkinson’s disease. From the results of neuroprotective study, all synthesized compounds showed good activity against 6-OHDA induced neurotoxicity at 31.25ug/ml concentrations and compounds VII-a (75.13), VII-b (74.91) and VII-h (72.01) possessed highest % cell viability. Compound VII-c has shown highest percentage of free radical scavenging activity with an of IC50 of 10.10µM.