In these research article reports The synthesis, characterization and antimicrobial activity of Spiro derivatives, namely N-(3-Alkoxyhydroxy-2-oxo-1-azaspiro[3.5]nonan-1-yl) isonicotinamide (6a-c). Spiro derivatives (6a-c) were synthesized by reacting N-(3-chloro-2-oxo-1-azaspiro[3.5]nonan-1-yl)isonicotinamide (4) with various hydroxy group containing drugs (5a-c). 2-Azetidinone derivatives, Compound (4) was synthesized by reacting Schiff base compounds, N'-cyclohexylidene isonicotino hydrazide (3) with chloro acetyl chloride. The Schiff base compounds, N'-cyclo hexylideneisonicotinohydrazide (3) was synthesised from isonicotino-hydrazide (1) and cyclochexanone (2). The synthesized compounds were characterized by using advanced analytical tools like NMR spectroscopy, IR spectroscopy and by Mass spectroscopy. All the newly synthesized compounds were evaluated for their Antibacterial activity.