Herein we report synthesis of 3-methyl-2-((2-styrylphenoxy)methyl)-4-(2,2,2-trifluoroethoxy)pyridine derivatives (9a-j) from 2-((3-methyl-4-(2,2,2-trifluoroethoxy)pyridin-2-yl)methoxy)benzaldehyde (7) and appropriate acetophenone (8a-f) afford very good to excellent yield (80-95 %). The structure of newly synthesized compounds have been established on the basis of 1H-NMR, 13C-NMR, ESI-MS and elemental analysis. All compounds (9a-f) were screened for their in vitro antibacterial activity against Gram-positive bacteria (Streptococcus pneumoniae and Bacillus subtilis), Gram-negative bacteria (Escherichia coli and Vibrio cholerae) and antifungal activity against C. albicans and A. fumigatus by MIC method.