Bio-based monomers and polymers have been of interest for the synthesis of new biomaterials owing to their low carbon footprint and wide availability. Among the vegetable oil-based polymers, Bio-epoxy resins obtained from vegetable oils are the most important polymer. In the present study, bioepoxy resin was synthesized from epoxidized cottonseed oil (ECO) and evaluated for potential wound-healing activity. The cottonseed oil was subjected to epoxidation using hydrogen peroxide and acetic acid. Bioepoxy resins were prepared using citric acid (CA) and tartaric acid (TA) acting as biobased curing agents. Four bioepoxy resin films (A-D) were prepared using ECO, CA, TA and ofloxacin (OFL). Evaluation of various physicochemical parameters i.e. folding endurance, surface pH, swelling index, moisture absorption, water permeation and mechanical properties showed that the bioepoxy resins are suitable for use as wound healing dressing. The Wound healing potential was screened using excision wound model on Wistar albino rats. All the animal groups treated with bioepoxy resins (A-D) showed significant improvement (p<0.05) in the percentage wound contraction rate and time of epithelisation as compared to control. Group III treated with film D (ECO + TA + OFL) demonstrated highest rate of wound contraction (7.4% to 53.72%) and shortest time for epithelization (5.66 + 0.22 days). Histopathological investigation of wound tissues samples revealed that groups treated bioepoxy resins had significant granulation, remodelling and contraction with horizontally arranged fibrous connective tissue. Thus, bioepoxy resin film dressings of prepared from epoxidized cottonseed oil can serve as an alternative for wound dressing films in the treatment of excision wounds.