Carbon nanoparticles are utilized in numerous medicinal and scientific procedures due to its low toxicity, biocompatibility and easy availability. They are synthesized using physical and chemical methods which involves consumption of more energy or usage of toxic chemicals. Hence, an alternate to synthesize carbon nanoparticles was evaluated using open pyrolysis and generation of soot. As the soot are generated from biological material in this study, i.e. Chromolaenaodorata, they are termed as Biosoot. Biosoot is defined as the smoke or soot generated when burning the biomaterials which may either be of plant or of animal origin. These soots are found to differ in their characteristic features as size,