Heterocyclic compounds have occupied a prominent place among various classes of organic compounds by virtue of their diverse biological activities and chemistry. Pyridine is important pharmacophores in modern drug discovery. Among pyridine skeleton occupies a significant place in numerous bioactive molecules with wide range of activities like anti microbial, anticancer, analgesic, anti-inflammatory and anticonvulsant the aim is to synthesize a drug with better efficacy, less toxicity and fewer side effects. In view of the important biological activities in the present work an attempt has been made to synthesize some six novel pyridine Schiff base derivatives (AZ-1-AZ6) and evaluated the biological profile of these compounds. The title compounds were evaluated for anti-TB activity using Microplate Alamar Blue Assay method, from the results revealed that these derivatives shown potent anti-TB activity when compare to standards pyrazinamide, streptomycin and isoniazid respectively