Multicomponent reaction of 2-(8-bromo-12-oxoindolo[2,1-b]quinazolin-6(12H)-ylidene) malononitrile and azomethine ylides formation from substituted aldehyde and α- amino acids (Proline and Sarcosine) to gives series of 8-bromo-12-oxo-3'-subtituted phenyl-5',6',7',7a'-tetrahydro-1'H,3'H,12H-spiro[indolo[2,1-b] quinazoline-6,2'-pyrrolizine]-1',1'-dicarbonitrile and 8-bromo-1'-methyl-12-oxo-2'-subtituted phenyl-12H-spiro[indolo[2,1-b]quinazoline-6,3'-pyrrolidine] -4',4'-dicarbonitrile in a quantitative yield. These compounds were screened for their anti-bacterial, anti- fungal and anti- mycobacterial activities. Best results were found in 8-bromo-3'-(4-fluorophenyl)-12-oxo-5',6',7',7a'-tetrahydro-1'H,3'H,12H-spiro[indolo[2,1-b] quinazoline -6,2'-pyrrolizine]-1',1'-dicarbonitrile and 8-bromo-2'-(4-fluorophenyl)-1'-methyl-12-oxo-12H-spiro[indolo[2,1-b]quinazoline-6,3'-pyrrolidine]-4',4'-dicarbonitrile with minimum inhibitory concentration (MIC) of 0.6 μg/ml against MTB.