The current study was based upon the synthesis of derivatives of substituted 2-(4-Bromophenyl)-1h-Benzo [D]Imidazole and the evaluation for antidepressant effect of the synthesized benzimidazole derivatives. The synthesized derivatives were evaluated for melting point determination, TLC, infrared, mass, NMR and Docking. Albino rats of either sex weighing 130-160g were obtained from the Animal House, Department of Pharmacy, IIMT University, Meerut (UP) India. The animals are maintained in proper conditions, at room temperatures of 25 ± 1°C with 12-hour light/dark cycle. Rats were divided into 4 group (n=6) and treated for 21 days i.e., group 1 was administered normal saline, group 2 administered fluoxetine (20mg/kg, orally), group 3 and group 4 administered all the novel derivatives of benzimidazole at 100mg/kg, orally and 200mg/kg, orally, respectively. Thin layer chromatography is used in synthetic chemistry to confirm the production of a molecule based on its Rf value, which varies depending on the compound. Rf value was obtained as 0.79, 0.73, 0.71, 0.83, and 0.76 of C1, C2, C3, C4, and C5, respectively. Antidepressant activity was evaluated through parameters including Tail Suspension Test, Motor coordination test and Locomotion Activity. In results, it showed that mass spectroscopy showed near molecular weight as estimated. TST and Rota-rod models exhibited highest level of decrease in time of motion that indicated for their anti-depressant potential. Actophotometer also shown moderate anti-depressant action while in the models, effect was observed in dose dependent manner. In conclusion, depression can be treated using synthesized substituted benzimidazole derivatives, which alleviate symptoms and boost mental health to help you in elevated mood. Its mode of action must be explained in detail before it can be considered for treatment of various mental health problems.