New Schiff base derivatives of benzothiazole 2(a-d) were synthesized through the reaction of 6-methoxy-1,3-benzothiazol-2-amine 1 with various substituted salicylaldehyde (a-d). Determination of compound structures was achieved through the analysis of infrared (IR), proton and carbon-13 nuclear magnetic resonance (1H/13C NMR), and ultraviolet (UV) spectral data. All the recently synthesized compounds underwent screening for their in vitro antibacterial effectiveness against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and B. subtilis. Additionally, they were assessed for their antifungal activity against Candida albicans, and Saccharomyces cerevisiae. Compounds 2b, and 2c demonstrated superior in vitro antibacterial and antifungal efficacy compared to the standard drugs.