Iron oxide nanoparticles are now widely used in biomedical applications because they are non-toxic to biological systems. Iron oxide nanoparticles' magnetic and semiconductor capabilities can also lead to multiple medical applications. The antibacterial, antifungal, and anticancer properties of these nanoparticles have been employed in the medical field. The diagnosis of cancer has been improved with the use of medicines and iron oxide nanoparticles for treatment. However, a lot of these medications have negative health impacts. As an alternative, plant-derived phytochemicals have been administered to functionalize these nanoparticles in order to counteract any unfavourable effects. In the present study, ex-situ co-precipitation was used to produce Fe3O4 nanoparticles that were then coated with Chitosan. Iron ferric and ferrous solutions were dissolved in ammonia to create Fe3O4 nanoparticles. Fe3O4 was synthesized prior to the addition of Chitosan. By using XRD, FTIR, SEM, UV-Vis methods, thermal (TGA) and magnetic (VSM) properties of nanoparticles with drug-loaded curcumin were identified.Then the samples’ anti-microbial activities are determined by using the gram positive and gram negative bacteria. The results clearly prove the structural, strength and anti-microbial activity of ironoxide/chitosan/curcumin loaded nanoparticlesHence, thisnanoparticlescan be used as a drug in near future.