Organic substances known as "schiff bases" have a wide range of biological activity, which can be enhanced by coordinating with various metal ions. Their compounds can even function as heterogeneous catalysts in chemical processes or as corrosion inhibitors. This work concentrated on the Schiff base generated from 2-amino-4-hydroxy-6-methyl pyrimidine due to the versatility of the Schiff base synthesis and its numerous possible applications. With the help of acetoacetic ester and guanidine, 2-amino-4-hydroxy-6-methylpyrimidine was successfully synthesized. Treatment of Phenylisothiocyanate with 2-amino-4-hydroxy-6-methylpyrimidine followed to yield 1-(4-hydroxy-6-methylpyrimidino)-3-phenylthiocarbamide. The structure of the Schiff base ligand has been synthesized and characterized UV–visible, FTIR, 1H NMR spectra, 13C NMR spectra and thermal analysis and screened for antidiabetic and antimicrobial activity. The biological activity result and the spectroscopic data verified Schiff base production, demonstrating the antidiabetic, and antibacterial properties of 1-(4-hydroxy-6-methylpyrimidino)-3-phenylthiocarbamide.