To evaluate the antibacterial and antifungal benefits of iron nanoparticles (FeNP) on an assortment of pathogenic microorganisms, we extracted the aqueous flower extract of Tabebuia rosea. The ratio of precursors to flower extraction was 1:1. Different concentrations of iron sulfate and flower extraction were employed. The findings revealed dependencies on the synthesis conditions and FeNP particle size. because there is a direct correlation between the circumstances of production along with a rise or reduction of the antimicrobial activity, the activity of the antimicrobial was then assessed against microbes in their Finally, the total flavonoids and overall polyphenols were quantified, as well as the antioxidant capacity of the Tabebuia rosea extract was determined, enabling the antimicrobial activity of the FeNPs to be linked to the extract's constituents and also docking Alpha-amylase glucosidase Frequency of amino acid and Fe 3+ & Fe2+ binding affinity was calculated.