Background &Method: The aim of the study is to find association between serum triglycerides and serum HNF1α with oxidative stress markers in type 2 diabetes patients. The microplate provided in this kit has been pre-coated with an antibody specific to HNF1α. study was conducted in the department of Biochemistry at the Sanotsh medical college & Hospital, Ghaziabad (U.P.). It is an observational case control study. Over 150 individuals from the Outpatient Department of Medicine at the Sanotsh Medical College in Ghaziabad participated in the current study. Out of 150 subjects selected, 75 were of T2DM, and 75 Normal healthy subjects which served as control group Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to HNF1α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Result: In our study we found 66.6% male & 33.4% female. In our study we found maximum no in age group of 51-60 (33.3%), followed by 41-50 (30.6%). Mean+SD in BMI kg/mt2 25.1725±5.34854. Mean+SD in HNF1A ng/ml 2.9943±1.89860. The chi-square statistic is 161.2834. The p-value is < 0.00001. The result is significant at p < .05.