Nitrogen-based compounds 4(3H)-quinazolinone and imidazole have a heterocyclic skeleton and a variety of biological functions, such as fungicidal, anticonvulsant, antibacterial, anticancer, antimalarial, and anti-inflammatory properties. The research into novel therapeutics based on the quinazolinone containing Imidazolium-based Metal N-Heterocyclic Carbene analogues core accelerated. The most significant breakthroughs in the synthesis of quinazolinone containing Imidazolium-based Metal N-Heterocyclic Carbene analogues, a scaffold that is highly valued for its therapeutic potential in the treatment of a number of ailments produced a range of promising outcomes. Novel quinazolinone-containing Imidazolium-based Metal N-Heterocyclic Carbene analogues have been produced, spectrum data was used to characterize it, and they have been investigated for their effectiveness as antibacterial agents against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa, as well as their antifungal activity against Candida albicans and Aspergillus niger. However, the same group of compounds also demonstrated strong in vitro antifungal activity against Candida albicans and Aspergillus niger. Compounds TM4a and TM4g showed good antibacterial activity in vitro against S. aureus, B. subtilis, E. coli, and P. aeruginosa.