Pediatric patients often exhibit fear, anxiety, and resistance towards undergoing laboratory tests, which can lead to inaccurate results and delayed diagnoses. Various nursing interventions have been developed to address these challenges and improve compliance among pediatric patients. This review critically examines the effectiveness of different nursing strategies, such as distraction techniques, play therapy, education, and emotional support, in encouraging pediatric patients to cooperate during laboratory testing. Additionally, the impact of nurse-patient communication, trust-building, and family involvement on enhancing compliance with testing procedures is discussed. The review also highlights the importance of tailoring interventions to the developmental stage and individual needs of pediatric patients to achieve optimal outcomes. Overall, this article emphasizes the crucial role of nurses in promoting pediatric patient compliance with laboratory testing and provides insights into best practices for improving the testing experience for young patients.