Background: Infection control practitioners (ICPs) are a group of specialized nurses fundamental to effective healthcare infection prevention and control initiatives. Much less is known about their working conditions relative to other groups of nurses. Organizational factors may impact ICPs’ levels of job dissatisfaction and emotional job burnout and, subsequently, their quality of practice. The aim of this study: is to measure a range of organizational factors to document the working conditions of ICPs and show how these are linked to job satisfaction and emotional burnout among ICPs. Methods: the study conducted a cross-sectional study using an online survey. All employed ICPs in 50 of the largest public hospitals in KSA were invited to participate. 153 ICPs completed the survey. Results: ICPs are moderate to highly satisfied with their job but show high levels of emotional burnout, time pressure, and cognitive demands. Low job satisfaction was associated with less job control, low perceived organizational support, and poor communication. In contrast, emotional burnout was associated with high time pressure and cognitive demands coupled with poor communication. Conclusions: Organizational context and factors are important to consider when evaluating the impact and implementation of infection control programs.