This article reports on the conceptual and empirical development of an instrument to measure life hardiness and spirituality, intended explicitly for studies of health. Progress in the study of health relations was hindered by the lack of an adequate measure of personality qualities and spirituality The aim of the study was to investigate whether there is a relationship between the spirituality of the personality and the life hardiness, the level of stress and the factors of the biopsychosocial mental model, which outlines the "self-concept" of the personality The scope of this study covered several questions: 1. Is there a statistically significant relationship between life hardiness and biopsychosocial model factors? 2. Is there any relationship between spirituality and other factors of the biopsychosocial-spiritual model?3. Is there a relationship between spirituality and stress level?4. Are there statistically significant differences between age and gender groups when analyzing spirituality, stress level and biopsychosocial-spiritual model factors? Total of 140 people, aged 18 to 80 (Mdn = 39; SD=7,05) participated in the study. By gender: 66 men and 74 women. By age group: 18-25 years – 48 participants, 26-50 years – 54 participants, 50-80 years – 38 participants. In order to conduct research Life Hardiness Test (Maddi, 2000,), “Biopsychosocispiritual Inventory” (Biopsychosocispiritual Inventory (BioPSSI); Katerndahl D., Oyiriaru D., 2007; Spiritual Assessment Scale; Hatch, R. L., Burg, M. A., Naberhaus, D. S., Hellmich, L. K., 1998 and how satisfied with life-stress level Walter W.Hudson, 2002) ,were used. Data was processed in Microsoft Excel and SPSS program. Work carried out in humanistic-systemic perspective where the development of spirituality is humane individual self-realization basis. Results demonstrated that there is statistically significant and close relation between internal life hardiness and stress level,spritual and factors of Biopsychosocispiritual model, statistically significant and close correlation between spirituality and factors of Biopsychosocispiritual model, statistically significant and close relation between spirituality and self-concept. It was also discovered that there is no statistically significant difference between male and female groups when analysing spirituality and factors of Biopsychosocispiritual model.