The present study was carried out to investigate the toxic effects of fluoride on the brush border membrane enzymes in kidney of rats and also to evaluate the ameliorative effect of Boerhaavia diffusa L.Eight groups of six rats each were randomly selected from a total of forty-eight animals. Rats in Control I were given deionized water orally. For 40 days, rats in the first and second groups received daily doses of 300 and 600 mg NaF/kg bw, respectively. For 20 days, 500 mg/kg b.w./day of Boerhaavia diffusa L. leaf extract was given orally to Control II rats. After receiving a 20-day pretreatment of 500 mg/kg bw/day of Boerhaavia diffusa L. leaf extract, groups third and fourth were subjected to 40 days of exposure to 300 and 600 mg NaF/kg bw/ /day. Groups fifth and sixth were exposed firstly to 300 and 600 mg NaF/ kg bw /day and then post-treated with leaf extract of Boerhaavia diffusa L. for 20 days. The renal cortex brush border membrane enzymes viz; acid (ACP) and alkaline phosphatase (ALP) , leucine aminopeptidase (LAP), γ-glutamyl transferase (γ-GGT) were analyzed .The activities of ALP,GGT, and LAP were significantly (P<0.001) declined , whereas activity of ACP was elevated in animals treated with 300 and 600mg NaF for 40 days. Pre and post treatment with leaf extract of Boerhaavia diffusa L. significantly restored (P<0.001) activities of these enzymes .Maximum decrease of (-48.14) ALP , (-58.97) γ-GGT , (-72.70) LAP , and maximum increase of (+482.29) ACP was recorded in rats exposed to 600 mg NaF in drinking water . The results of the present study show that the enzymatic alterations in the brush border membrane in the kidney of rats treated with sodium fluoride were considerably reduced by leaf extract of Boerhaavia diffusa L. both before and after the treatment.