The greatest challenge currently facing the field of dentistry and oral health is dental caries, which is caused by a number of interacting (multifactorial) factors, including elements outside the mouth that are indirectly related to dental caries, among others. Knowledge and techniques pertaining to the prevention of dental caries. The dissemination of dental health education using various educational media, such as booklets, is one method to increase the knowledge and practice of school-aged children in maintaining dental health, particularly in caries prevention. The purpose and benefit of this research are to produce a booklet that can be used in dental health education, particularly in preventing dental caries in elementary school students, and to assess the effect of the booklet on knowledge and practice in preventing dental caries. This research was conducted on fifth-grade students at the State Elementary School 03 Pondok Labu, South Jakarta. The type of research used is a quasi-experimental research design with a Non-randomized control group pre-test and post-test design. The study had a total sample size of 148 students, with 74 students in the treatment group and 74 students in the control group. Using the Wilcoxon Signed Rank and Mann-Whitney tests, we analyzed the data. The results demonstrated that the dental caries booklet and its prevention could be used as a medium for dental health education to increase the knowledge and practice of elementary school students regarding dental caries and its prevention, and that there is a significant relationship between the provision of the booklet media and increased knowledge (p-value = 0.006) and practice (pvalue = 0.006) and practice (p-value = 0.030) students in the effort to prevent dental caries.