Iodine is an essential trace element for normal growth and development of fetus body. Iodine deficiency disorder is one of the most important preventable micro nutrition deficiency problems globally. Aim: The aim of this study is to assess pregnant women’s Knowledge and attitude regarding iodine deficiency .Research design: A descriptive study design was utilized. Setting: This study was conducted at the Antenatal care clinic of Fayoum general hospital. Sample: A Convenience sample was utilized to recruit 100 pregnant women. Tools:. Three tools for data collection were used. First tool: A structured interviewing questionnaire to assess Socio demographic data, Medical, obstetric and menstrual ,current history. Second tool: Pregnant women's knowledge regarding Iodine deficiency. Third tool: Pregnant women's attitudes regarding Iodine deficiency. Result: The current study showed that less than three fifth of pregnant women having low level of knowledge regarding Iodine deficiency, while less than half of pregnant women had negative attitudes regarding Iodine deficiency during pregnancy Conclusion: the study pregnant women had unsatisfactory level of knowledge and negative attitudes regarding Iodine deficiency. Recommendation: Developing program to increase awareness regarding Iodine deficiency among pregnant women...