Tuberculosis (TB), a leading cause of mortality worldwide, is particularly rampant in Asia and Africa. India alone accounts for a fifth of the global TB cases annually. The rise of drug-resistant Mycobacterium tuberculosis strains (MDR and XDR) poses a significant challenge, especially in developing nations. This underscores the need for alternative treatments, such as plant-based drugs. This study presents a comprehensive phytochemical screening in extracts from Aloe vera, Ocimum tenuiflorum (Holy Basil), and Martynia annua L (Tiger's Claw) for their further anti-tubercular potential. Various solvent extracts were prepared and analyzed for secondary metabolites like Alkaloids, Flavonoids, Terpenoids, Steroids and Tannins, where earlier studies advocate the highest antimicrobial property in alkaloids and flavonoids. Using water and methanol extracts, we analyzed the phytochemicals of Aloe vera, Ocimum tenuiflorum, and Martynia annua L. We detected various phytochemicals in different extracts, such as alkaloids, flavonoids, terpenoids, steroids, and tannins. These phytochemicals have anti-tubercular and other pharmacological effects. Ocimum tenuiflorum showed the highest anti-tubercular potential with the most alkaloids and flavonoids in methanol extract. Aloe vera and Martynia annua L had similar phytochemicals, except for the absence of alkaloids in water extract of Aloe vera. These plants are used for various diseases, and our findings confirm their therapeutic value. This research highlights the potential of these plants as sources of anti-tubercular agents on the basis of their biochemical profile. The findings provide valuable insights into their bioactive components, paving the way for further investigations into novel anti-tubercular drugs based on these compounds