To find new, safe, and more effective drugs, the present study investigated antioxidant activity, In vitro antimicrobial activity, and phytochemical screening of different fractions (petroleum ether, chloroform, ethyl acetate, methanolic, and water) of Berberis aquifolium and Curcuma longa (fruit and rhizome). The antimicrobial activity of the different fractions of both plants was studied against ten (gram-negative and gram-positive) bacteria. The methanolic fraction of both plants fruit and rhizome parts exhibited potent in vitro antimicrobial activity. The methanolic fraction of Curcuma longa showed maximum zones of inhibition in these bacteria (24±1mm, 22±1mm, and 21±1mm) Staphylococcus aureus ˃ Staphylococcus epidermidis ˃ Escherichia coli. In phytochemical testing, carbohydrates, glycosides, terpenoids, flavonoids, alkaloids, phenols, resins, and tannins are found in plants.